Spectrofluorometer

Make &Model: Jasco, FP-8300

       The FP-8300 is a user friendly spectrofluorometer with a wide range of accessories that are well suited to biological research. The standard automatic band-pass filters obtain spectra without the artificial peaks due to second order diffracted light. The Auto-Gain and Auto-SCS functions optimize the S/N for samples with large differences in signal intensity and concentration. Temperature control accessories can be used for thermal melting studies, providing researchers with fluorescence and thermodynamic data for conformational and folding studies. FRET measurements offer insight on binding and folding dynamics. The FP-8300’s automatic polarizers can perform fluorescence anisotropy measurements to provide information regarding fluorophore mobility. Kinetic and titration measurements can also be performed with the dedicated stopped-flow and autotitrator systems

Features

• High sensitivity S/N > 2,800 (RMS)
• High resolution of 1.0 nm
• Wavelength range: 200 to 750 nm (900 nm optional)

Applications:

• Detection of Phosphorescence from Singlet Oxygen using NIR Spectrofluorometer
• High Sensitivity Fluorescence Detector for Supercritical Fluid Chromatography
• Temperature-Dependent CD and Fluorescence Spectral Measurements of Lysozyme
• CPL Measurement of Camphorquinone Using CPL-300 Circularly Polarized Luminescence Spectrometer
• CPL Spectrum Measurement of Green Fluorescent Protein (GFP)
• Internal Quantum Efficiency Measurement of Phosphor Powders
• Evaluation of FRET as a Sensitive Biosensor for Explosives
• Eliminating Fluorescence Artifacts from the Diffuse Reflectance Measurement of Sodium Salicylate
• Remote Measurement using FP-8500 Fluorometer with Optical Fiber
• Near-Infrared Phosphor Measurement
• Quantitative Analysis of a λ DNA Using a One Drop Accessory
• Activity Measurement of Trypsin Using a Fluorescence Peptide Substrate
• Absolute Quantum Yields Using an Integrating Sphere
• Quantum Efficiency of Phosphorescent Materials using an Integrating Sphere
• High-Speed Measurement and EEM Interpretation for Olive Oil Analysis
• Fluorescence Depolarization Measurement of Liposome
• Determination of the Relative Quantum Yield of Rhodamine B
• Quantum Yield Measurement of the Up-Conversion Phosphors
• Measuring the Fluorescence Anisotropy Spectrum of Rhodamine B
• High Sensitivity Coumarin Analysis
• Component Analysis of an Excitation-Emission Matrix of Water Samples Using PARAFAC Analysis
• Luminescent Color Evaluation for Fluorescent Materials
• Analysis of Fluorescence Materials at High Temperatures
• Fluorescence and CD Spectra using the Temperature/Wavelength Measurement
• Fluorescence Detection in the Detection of Counterfeit US Currency

Courses Offered: Diploma,Certificate